Diagnosis of rotavirus, adenovirus, and herpesvirus infections by immune electron microscopy using a serum-in-agar diffusion method
- 1 February 1980
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 26 (2) , 261-264
- https://doi.org/10.1139/m80-041
Abstract
The sensitivity of immune electron microscopy (IEM) for the detection and identification of bovine rotavirus, infectious bovine rhinotracheitis virus (IBR), and canine adenovirus has been studied by using the serum-in-agar (SIA) method in which a specific antiserum has been incorporated in agar.In comparison with standard electron microscopy, in which the viral suspensions are used undiluted, this method permits the detection of viral particles either isolated or in aggregates contained in suspensions diluted up to 100 times in the case of rotavirus and adenovirus, and up to 10 times for the herpesvirus suspensions.Keywords
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