HL-A LD (Lymphocyte Defined) Typing: A Rapid Assay with Primed Lymphocytes

27 June 1975

journal article
other
Published by American Association for the Advancement of Science (AAAS) in Science

Vol. 188 (4195) , 1308-1310
https://doi.org/10.1126/science.124948

Abstract

When human lymphocytes are cultured for 9 to 14 days with stimulating cells of a family member differing by a single HL-A haplotype they become "primed" to recognize specific HL-A LD (mixed lymphocyte culture) antigens. These primed lymphocytes respond specifically and rapidly when "restimulated" with cells of a person that contain the same LD antigens as those of the priming haplotype. Specific HL-A LD antigens can be detected within 24 hours by this primed LD typing.

Keywords

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