Phorbol esters inhibit inositol phosphate and diacylglycerol formation in proliferating HL60 cells Relationship to differentiation
- 20 June 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 233 (2) , 239-243
- https://doi.org/10.1016/0014-5793(88)80434-4
Abstract
Phorbol 12‐myristate 13‐acetate (PMA) induces the differentiation of the human promyelocytic cell line, HL60, towards adherent macrophage‐like cells within 2 days. We have examined the early effects of PMA on inositol phosphates and on diacylglycerol production, two second messengers derived from inositol lipids. In proliferating HL60 cells, PMA induced a time‐ and concentration‐dependent decrease in inositol phosphate levels. Maximal effects were seen after 1 h at 10 nM PMA. PMA also induced the translocation of protein kinase C from the cytosol to the membrane. Comparison between the differentiating effects of several phorbol esters and of 1‐oleoyl‐2‐acetylglycerol with their ability to inhibit inositol phosphate formation suggests that the two effects are correlated.Keywords
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