Measurement of Serum Lactic Dehydrogenase
- 1 June 1961
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 7 (3) , 265-274
- https://doi.org/10.1093/clinchem/7.3.265
Abstract
A colorimetric method is described for the measurement of serum lactic dehydrogenase (LDH) activity. The method employs phenazine methosulfate to mediate electron transfer between reduced diphosphopyridine nucleotide and 2,6-dichloroindophenol, and allows continuous measurement of the reaction rate in the direction lactate to pyruvate. The LDH of a number of normal and pathologic sera was measured by the present method and by the ultraviolet spectrophotometric method. Agreement between the two methods is good.Keywords
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