Analysis of the 16S rRNA Gene Sequence ofAnaplasma centraleand Its Phylogenetic Relatedness to Other Ehrlichiae
Open Access
- 1 March 2001
- journal article
- research article
- Published by American Society for Microbiology in Clinical and Diagnostic Laboratory Immunology
- Vol. 8 (2) , 241-244
- https://doi.org/10.1128/cdli.8.2.241-244.2001
Abstract
The nucleotide sequence of the Anaplasma centrale 16S rRNA gene was determined and compared with the sequences of ehrlichial bacteria. The sequence of A. centrale was closely related to Anaplasma marginale by both level-of-similarity (98.08% identical) and distance analysis. A species-specific PCR was developed based upon the alignment data. The PCR can detect A. centrale DNA extracted from 10 infected bovine red blood cells in a reaction mixture. A. centrale DNA was amplified in the reaction, but not other related ehrlichial species.Keywords
This publication has 33 references indexed in Scilit:
- Detection of ehrlichiae in African ticks by polymerase chain reactionTransactions of the Royal Society of Tropical Medicine and Hygiene, 2000
- Tree View: An application to display phylogenetic trees on personal computersBioinformatics, 1996
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Protein Analysis of Anaplasma marginale and Anaplasma centrale by Two-Dimensional Polyacrylamide Gel Electrophoresis.The Journal of Veterinary Medical Science, 1994
- Identification of immunodominant polypeptides common between Anaplasma centrale and Anaplasma marginaleVeterinary Immunology and Immunopathology, 1991
- Analysis of protein compositions and surface protein epitopes of Anaplasma centrale and Anaplasma marginale.The Journal of Veterinary Medical Science, 1991
- Recognition of conserved surface protein epitopes on Anaplasma centrale and Anaplasma marginale isolates from Israel, Kenya and the United statesInternational Journal for Parasitology, 1988
- Enzyme-linked immunosorbent assay for detection of antibodies to Anaplasma centrale.The Japanese Journal of Veterinary Science, 1988
- Immunization with an Isolate-Common Surface Protein Protects Cattle Against AnaplasmosisScience, 1986
- A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequencesJournal of Molecular Evolution, 1980