Demonstration of Birch Pollen Allergen from Isolated Pollen Grains Using Immunofluorescence and a Single Radial Immunodiffusion Technique

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Abstract
Immunofiuorescence (IF) and single radial immunodiffusion (SRID) methods were modified in order to study the relation of allergen to the surface of birch pollen. By absorption of the anti-birch pollen sera employed, it was ascertained that both methods demonstrated thermostable allergenic birch pollen antigen and not nonallergenic antigens. The allergen rapidly diffuses from unfixed, wetted grains. After an aldehyde treatment and wet heating of the grains this leakage which mainly occurs through the pollen apertures was not demonstrated. Birch pollen allergen was released in a similar way from pollen of all of the six tested species of birch.

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