Specific increase of a 35K protein in the sera of cows with fatty liver.

Abstract
Sera and liver extracts of cows with fatty liver were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis to demonstrate the specific changes in protein profile of fatty liver cows. In the sera from cows with fatty liver, a 35K protein was found to be increased as compared to that of sera from normal cows. On the other hand, the increase of 35K protein was not observed in sera from cows with other diseases we so far tested. The increase of 35K protein in sera from fatty liver cows was further confirmed by gel filtration of the sera using Sephadex G-100. The increase of 35K protein was also observed in liver extracts from fatty liver cows. These results suggested that the increase of this protein was specifically associated with fatty liver of cows.

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