SECRETION OF ERYTHROPOIETIN-LIKE ACTIVITY BY CLONES OF HUMAN RENAL-CARCINOMA CELL-LINE GKA

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (1) , 51-54
Abstract
Human renal carcinoma cell line GKA was derived from a patient with the paraneoplastic syndrome of erythrocytosis and secretes erythropoietin-like activity into its growth medium (Sytkowski, A.J., Richie, J.P., and Bicknell, K.A., 1983). To derive homogeneous sublines with higher secretory rates, line GKA was cloned. Over 100 clones were generated, and 21 secreted erythropoietin-like activity, up to 6-fold higher than the uncloned line. This activity stimulated the growth and differentiation of CFU-E [erythroid colony-forming unit] derived colonies in plasma clot culture. The secreted erythropoietin-like activity did not cross-react in a sensitive radioimmunoassay utilizing highly purified 125I-labeled human urinary erythropoietin and heterologous anti-human urinary erythropoietin antiserum. Line GKA apparently secretes an erythropoietic stimulating factor distinct from the hormone erythropoietin.