Precise Centromere Mapping Using a Combination of Repeat Junction Markers and Chromatin Immunoprecipitation–Polymerase Chain Reaction
- 1 October 2006
- journal article
- research article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 174 (2) , 1057-1061
- https://doi.org/10.1534/genetics.106.060467
Abstract
Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.Keywords
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