A single-injection protein kinase A-directed antisense treatment to inhibit tumour growth
- 1 June 1995
- journal article
- research article
- Published by Springer Nature in Nature Medicine
- Vol. 1 (6) , 528-533
- https://doi.org/10.1038/nm0695-528
Abstract
Expression of the RIα subunit of cAMP-dependent protein kinase type I is enhanced in human cancer cell lines, in primary tumours, in cells after transformation and in cells upon stimulation of growth. We have investigated the effect of sequence-specific inhibition of RIα gene expression on in vivo tumour growth. We report that single injection RIα antisense treatment results in a reduction in RIα expression and inhibition of tumour growth. Tumour cells behaved like untransformed cells by making less protein kinase type I. The RIα antisense, which produces a biochemical imprint for growth control, requires infrequent dosing to halt neoplastic growth in vivo.Keywords
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