Adsorption of Tetralkyl Ammonium Ions on Reversed-Phase HPLC Columns

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Abstract
A systematic study of the influence of several chromatographic parameters upon adsorption of tetralkyl ammonium ions on reversed-phases has been made. Results show that organic solvent content in mobile phase, tetralkyl ammonium hydrophobicity and tetralkyl ammonium concentration in eluent are the most influent variables. A mathematical expression to calculate the adsorbed ion concentration for different combinations of these variables is deduced. Results from this study can be applied in Ion Pair Reversed-Phase Chromatography to calculate column equilibration times, for setting gradient elution separation methods and for the study of retention mechanisms.