An Improved Method for Heterozygote Identification in Feline and Human Mucopolysaccharidosis VI, Arylsulfatase-B Deficiency
- 1 July 1981
- journal article
- research article
- Published by S. Karger AG in Enzyme
- Vol. 26 (4) , 206-210
- https://doi.org/10.1159/000459176
Abstract
An improved method has been developed for the detection of heterozygotes for feline and human mucopolysaccharidosis VI. Arylsulfatase-A and -B activities were assayed in leukocyte extracts following separation of the enzymes by batch chromatography on DEAE cellulose. Determination of arylsulfatase-B specific activities did not permit accurate heterozygote identification, whereas the arylsulfatase-A to arylsulfatase-B activity ratio discriminated all 16 obligate heterozygotes for the feline and human disorders.Keywords
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