Direct evidence of a heterotrimeric complex of human interleukin-4 with its receptors
Open Access
- 1 March 1995
- journal article
- research article
- Published by Wiley in Protein Science
- Vol. 4 (3) , 382-386
- https://doi.org/10.1002/pro.5560040304
Abstract
The mode of binding of interleukin-4 (IL-4) to its two known receptors, specific receptor IL-4R and a shared receptor γc, was investigated using gel filtration and gel electrophoresis. A ternary complex between IL-4 and the soluble domains of the two receptors was shown to exist in solution. The association constant between γc and the stable complex of IL-4/sIL-4R is in the millimolar range, making the ternary complex a feasible target for crystallization studies.Keywords
This publication has 38 references indexed in Scilit:
- Stoichiometry of the complex of human interleukin‐4 with its receptorFEBS Letters, 1994
- Cloning of a T Cell Growth Factor that Interacts with the β Chain of the Interleukin-2 ReceptorScience, 1994
- Characterization of the interleukin 4 receptor. Structure and signal transduction pathwaysResearch in Immunology, 1993
- Interleukin-2 Receptor γ Chain: a Functional Component of the Interleukin-4 ReceptorScience, 1993
- Interleukin-2 Receptor γ Chain: a Functional Component of the Interleukin-7 ReceptorScience, 1993
- Hematopoietin sub-family classification based on size, gene organization and sequence homologyCurrent Biology, 1993
- Crystal structure of human recombinant interleukin‐4 at 2.25 Å resolutionFEBS Letters, 1992
- Human interleukin 4: The solution structure of a four-helix bundle proteinJournal of Molecular Biology, 1992
- Haemopoietic receptors and helical cytokinesImmunology Today, 1990
- Serological, biochemical, and functional identity of B cell-stimulatory factor 1 and B cell differentiation factor for IgG1.The Journal of Experimental Medicine, 1985