Oligomer formation of histamine H2 receptors expressed in Sf9 and COS7 cells1

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Abstract
A histamine H2 receptor, which had been mutated at its glycosylation site and tagged at its N‐terminus with an HA tag (HA‐H2 receptor), was expressed in Sf9 cells and COS7 cells. Immunoprecipitation and immunoblotting of HA–H2 receptors with αHA antibody revealed four bands of 31.5±2.5 kDa, 59.0±6.0 kDa, 80.5±4.5 kDa and 120 kDa. These bands were also detected by immunoblot using anti‐H2 receptor serum (C‐terminus). In addition, H2 receptors without the HA‐tag co‐immunoprecipitated with HA‐tagged H2 receptors devoid of the 51 C‐terminal amino acids, via immunoprecipitation with αHA antibody, when the two receptors were coexpressed. These results suggest that H2 receptors are present as receptor oligomers and that the C‐terminal portion is not involved in the formation of these oligomers.