Degradation of 2-bromo-, 2-chloro- and 2-fluorobenzoate by Pseudomonas putida CLB 250

15 July 1989

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 60 (2) , 143-147
https://doi.org/10.1016/0378-1097(89)90497-7

Abstract

Pseudomonas putida strain CLB 250 (DSM 5232) utilized 2-bromo-, 2-chloro- and 2-fluorobenzoate as sole source of carbon and energy. Degradation is suggested to be initiated by a dioxygenase liberating halide in the first catabolic step. After decarboxylation and rearomatization catechol is produced as a central metabolite which is degraded via the ortho-pathway. After inhibition of ring cleavage activities with 3-chlorocatechol, 2-chlorobenzoate was transformed to catechol in nearly stoichiometric amounts. Other ortho-substituted benzoates like anthranilate and 2-methoxybenzoate seem to be metabolized via the same route.

Keywords

2-CHLOROBENZOATE
2-BROMOBENZOATE
2-FLUOROBENZOATE
CATECHOL
BENZOATE-1
2-DIOXYGENASE
PSEUDOMONAS PUTIDA

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