Evaluation of Mrna Markers for the Identification of Menstrual Blood
- 1 November 2002
- journal article
- Published by ASTM International in Journal of Forensic Sciences
- Vol. 47 (6) , 1278-1282
- https://doi.org/10.1520/jfs15560j
Abstract
The detection of epithelial cells in dried bloodstains by reverse transcriptase-polymerase chain reaction is based on cell- and tissuespecific gene expression. In this paper mRNA markers suitable for the identification of menstrual blood were evaluated. RNA isolated from autopsy tissue samples including endometrium, vaginal mucosa, and blood were screened for tissue-specific expression patterns using RT-PCR with primers for hormone receptors, intermediate filaments, matrix metalloproteinases, heat shock proteins, cytokines, and growth factors. Matrix metalloproteinase (MMP) mRNA could be detected in endometrium but not in blood and other epithelia. This was confirmed in further studies with artificial menstrual bloodstains, indicating that the detection of MMP expression in bloodstains may serve as a forensic marker for menstrual blood.Keywords
This publication has 19 references indexed in Scilit:
- An Inverse Relation Between the Expression of Tumor Necrosis Factor Alpha (TNF‐α) and TNF‐α Receptor in Human EndometriumAmerican Journal of Reproductive Immunology, 1999
- Oestrogen receptor alpha and mRNA expression in human endometrium throughout the menstrual cycleMolecular Human Reproduction, 1999
- Matrix Metalloproteinases (MMPs) and Their Physiological Inhibitors (TIMPs) Are Differentially Expressed during Excisional Skin Wound RepairExperimental Cell Research, 1998
- Homologous Down-Regulation of Luteinizing Hormone/Chorionic Gonadotropin Receptors by Increasing the Degradation of Receptor Transcripts in Human Uterine Endometrial Stromal CellsBiology of Reproduction, 1997
- The signals and molecular pathways involved in human menstruation, a unique process of tissue destruction and remodellingMolecular Human Reproduction, 1996
- Patterns of matrix metalloproteinase expression in cycling endometrium imply differential functions and regulation by steroid hormones.Journal of Clinical Investigation, 1994
- Expression of Leukemia Inhibitory Factor in Human Endometrium and Placenta1Biology of Reproduction, 1994
- Reverse Transcription-Polymerase Chain Reaction Phenotyping of Metalloproteinases and Inhibitors Involved in Tumor Matrix InvasionDiagnostic Molecular Pathology, 1993
- The identification of menstrual blood — the immunoelectrophoretic characterisation of soluble fibrinogen from menstrual bloodstain extractsForensic Science, 1974
- Identification of menstrual blood stains by the electrophoretic pattern of lactate dehydrogenase isozymesForensic Science, 1972