Oxygen evolution in dark-developed spruce chloroplasts

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Abstract
Chloroplasts isolated from dark-grown seedlings of Picea abies showed activities of DCIP and Fecy photoreductions widiout addition of an electron donor to PS-II. In addition to this, when light-induced oxygen evolution was measured with an oxygen electrode, a significant amount of oxygen was found. These results indicate that the photoreductions are coupled to the oxygen-evolving reaction. Furthermore, thylakoid membranes were functional in the proton uptake and the 515-nm absorbance change as parameters of their physicochemical functions. Electron microscopy showed that thylakoids were well-developed with prolamellar bodies and partially stacked to from grana. We conclude that oxygen-evolving ability and the physicochemical function of thylakoid membranes develop in chloroplasts of dark-grown spruce seedlings.

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