EFFECT OF D-GALACTOSAMINE ON PLASMA-PROTEIN SYNTHESIS BY PERFUSED RAT-LIVER FROM TURPENTINE-STIMULATED DONORS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 59  (5) , 504-513
Abstract
D-galactosamine (100 mg) was added to the reconstituted blood during 4 h perfusion of livers isolated from control rats or those injected with turpentine 20 h or 5 earlier. This galactosamine dose administered 30 min before [3H]lysine significantly inhibited the incorporation of the label into liver proteins, and even more into plasma proteins, but albumin and acute-phase reactants (fibrinogen, seromucoid fraction, concanavalin A-adsorbed glycoproteins) were all similarly affected. When galactosamine was administered in vivo simultaneously with turpentine and the liver was isolated 5 h later, trauma-induced fibrinogen synthesis was selectively inhibited. This can be explained either by a differential control of synthesis of various acute-phase reactants, or by augmentation of fibrinogen catabolism in galactosamine-treated rats. Crossed immunoelectrophoresis of the full perfusate or concanavalin A-adsorbed glycoproteins did not reveal any significant effect of galactosamine on the protein pattern obtained from control or turpentine-stimulated liver donors.