Molecular and biochemical characterization of dNOS: a Drosophila Ca2+/calmodulin-dependent nitric oxide synthase.
- 26 September 1995
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 92 (20) , 9072-9076
- https://doi.org/10.1073/pnas.92.20.9072
Abstract
Nitric oxide (NO) is an intercellular messenger involved with various aspects of mammalian physiology ranging from vasodilation and macrophage cytotoxicity to neuronal transmission. NO is synthesized from L-arginine by NO synthase (NOS). Here, we report the cloning of a Drosophila NOS gene, dNOS, located at cytological position 32B. The dNOS cDNA encodes a protein of 152 kDa, with 43% amino acid sequence identity to rat neuronal NOS. Like mammalian NOSs, DNOS protein contains putative binding sites for calmodulin, FMN, FAD, and NADPH. DNOS activity is Ca2+/calmodulin dependent when expressed in cell culture. An alternative RNA splicing pattern also exists for dNOS, which is identical to that for vertebrate neuronal NOS. These structural and functional observations demonstrate remarkable conservation of NOS between vertebrates and invertebrates.Keywords
This publication has 65 references indexed in Scilit:
- Induction of a dominant negative CREB transgene specifically blocks long-term memory in DrosophilaCell, 1994
- Endothelial NOS and the Blockade of LTP by NOS Inhibitors in Mice Lacking Neuronal NOSScience, 1994
- Nitric oxide mediates network oscillations of olfactory interneurons in a terrestrial molluscNature, 1994
- C/EBP is an immediate-early gene required for the consolidation of long-term facilitation in AplysiaCell, 1994
- A nitric oxide agonist stimulates consolidation of long-term memory in the 1-day-old chick.Behavioral Neuroscience, 1994
- Nitric oxide synthase activity from a hematophagous insect salivary glandFEBS Letters, 1993
- Structural Diversity of Neuronal Nitric Oxide Synthase mRNA in the Nervous SystemBiochemical and Biophysical Research Communications, 1993
- Particular Ability of Liver P450s3a to Catalyze the Oxidation of Nω-Hydroxyarginine to Citrulline and Nitrogen Oxides and Occurrence in NO Synthases of a Sequence Very Similar to the Heme-Binding Sequence in P450sBiochemical and Biophysical Research Communications, 1993
- Inhibition of nitric oxide synthesis impairs two different forms of learningNeuroReport, 1992
- Cloning and Characterization of Inducible Nitric Oxide Synthase from Mouse MacrophagesScience, 1992