PATTERNS OF URINARY-EXCRETION OF MODIFIED NUCLEOSIDES

  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (4) , 1150-1153
Abstract
High-performance liquid chromatography permits quantitative measurement of low levels of modified nucleosides in urine. The patterns of excretion of 7 modified nucleosides by normal subjects and cancer patients are reported. The excretion of these nucleosides expressed as a function of creatinine concentration was constant, not episodic nor related to diet. Randomly collected samples of urine are satisfactory sources for measurement of nucleosides, and the level of nucleosides is of significance when related to creatinine excretion. The constancy of the excretion of the modified nucleosides in normal subjects is quite remarkable. It implies strict metabolic control of tRNA turnover. The values for the individual nucleoside/creatinine ratios were significantly elevated in the urine of colon cancer patients.