Kinetics of the Treponema Immobilization Reaction Under Improved Conditions

Open Access
Publisher Website
Google Scholar
Abstract
A number of modifications of the TPI test aimed at lessening the tendency to clotting and lessening anti-complementary activity of the treponemal suspension are reported. Extraction of treponemes from the testicular tissue at lower temperature, separation from most of the testicular tissue juice, and resuspension in a modified Nelson''s medium permitted survival of 90% or more of treponemes in the controls for 48 hours. Sensitivity of the test varies with the concentration of complement and with the square of the reaction time. Toxic or anti-complementary sera may be satisfactorily tested, the authors state, by prolonging the incubation period and diluting the serum. Bio-assay, using a referance-standard serum, is recommended.