GENERATION OF CFU-C-SUPPRESSOR T-CELLS INVITRO - AN EXPERIMENTAL-MODEL FOR IMMUNE-MEDIATED MARROW FAILURE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 57  (3) , 491-496
Abstract
T cells were derived from the bone marrow of 8 healthy [human] donors and fractionated, according to their receptors for the Fc fragment of IgG, into TG+ [IgG Fc receptor-bearing] and TG- lymphocytes. These were then cocultured with autologous or allogeneic bone marrow cells in agar in the CFU-C [colony-forming cell] assay. No significant colony formation suppression was detected. Total T, TG+ and TG- cells were then incubated for 18 h with PWM [pokeweed mitogen], washed and cocultured with bone marrow cells. PWM-treated TG- cells showed no significant CFU-C suppressor activity; PWM-treated total T and TG+ cells inhibited colony formation of both autologous and allogeneic marrow cells. The supernatant of PWM-treated total T and TG+ cells also inhibited colony formation. PWM alone enhanced colony formation. Apparently normal T cells can be activated in vitro to become CFU-C/suppressor cells after PWM stimulation. This effect is evidently mediated by T cells with the Fc receptor for IgG.

This publication has 4 references indexed in Scilit: