Protection of avian myeloblastosis virus (AMV) DNA polymerase by substrates against heat inactivation
Open Access
- 1 November 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 1 (11) , 1399-1410
- https://doi.org/10.1093/nar/1.11.1399
Abstract
Purified DNA polymerase from AMV was exposed to thermal inactivation in the presence and absence of various compounds required for maximal activity. A synergistic protective effect was noted when all the components were present. The enzyme showed a distinct preference for primed templates, especially for its native 70S RNA. Heat inactivation studies also suggested that the active sites for RNase H and that for the polymerase were not identical.Keywords
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