Simultaneous determination of prednisolone and Cortisol in serum by HPLC and by isotope dilution—mass spectrometry

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Abstract
A reference method for the simultaneous assay of cortisol and prednisolone by isotope dilution-mass spectrometry was developed. A fixed amount of 2H4-labeled cortisol is added to a fixed amount of [human] serum. The mixture is then extracted, converted into methoxime-trimethylsilyl derivative, and subjected to analysis by combined gas chromatography-mass spectrometry. The ions at m/e [massicharge ratio] 603, m/e 605 and m/e 609 are followed through the gas chromatography. These ions correspond to the M-31 ions in the mass spectrum of methoxime-trimethylsilyl ether derivative of prednisolone, unlabeled cortisol and 2H4-cortisol, respectively. With the use of a standard curve, the concentration of prednisolone can be calculated from the ratio between the peak at m/e 603 and the peak at m/e 609, and the concentration of cortisol can be calculated from the ratio between the peak at m/e 605 and the peak at m/e 609. The coefficient of variation was .apprx. 2% with respect to determination of prednisolone and .apprx. 3% with respect to determination of cortisol. This method was compared with a routine method based on high-performance liquid chromatography (HPLC). The coefficient of variation was 2-3% with respect to determination of cortisol and 1-3% with respect to determination of prednisolone. There was a good correlation between the isotope dilution method (x) and the HPLC method (y). In the assay of serum prednisolone, the regression analysis gave the following equation: y = 1.02x -69 nmol/l, r = 0.99. In the assay of serum cortisol, the regression equation was y = 1.07x -14nmol/l, r = 0.95. Apparently, the HPLC method, used for routine determination of cortisol prednisolone, has an accuracy sufficient for its intended use.