Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
Open Access
- 1 November 2001
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (11) , 5331-5334
- https://doi.org/10.1128/aem.67.11.5331-5334.2001
Abstract
Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced intoRalstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB.Keywords
This publication has 33 references indexed in Scilit:
- Polyhydroxyalkanoate Degradation Is Associated with Nucleotide Accumulation and Enhances Stress Resistance and Survival ofPseudomonas oleovoransin Natural Water MicrocosmsApplied and Environmental Microbiology, 2001
- β-Ketothiolase genes in Azotobacter vinelandiiGene, 2000
- Metabolic routing towards polyhydroxyalkanoic acid synthesis in recombinantEscherichia coli(fadR): inhibition of fatty acid β-oxidation by acrylic acidFEMS Microbiology Letters, 1998
- Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: comparison of PhaC1 and PhaC2FEMS Microbiology Letters, 1997
- Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteriaFEMS Microbiology Letters, 1992
- Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteriaFEMS Microbiology Letters, 1992
- Cloning and molecular analysis of the poly(3‐hydroxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1European Journal of Biochemistry, 1992
- Shuttle vectors containing a multiple cloning site and a lacZα gene for conjugal transfer of DNA from Escherichia coli to Gram-positive bacteriaGene, 1991
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983
- Wide host range cloning vectors: A cosmid clone bank of an Agrobacterium Ti plasmidPlasmid, 1982