Expression of glycoprotein 90K in human malignant pleural mesothelioma: correlation with patient survival

Publisher Website
Google Scholar
Abstract
The expression of the tumour‐associated glycoprotein 90K in patients with malignant pleural mesothelioma (MM) has not been described. This study used enzyme‐linked immunoassay (ELISA) to measure 90K in pleural effusions (PEs) and sera from patients with MM (n=28), lung cancer (LC) (n=14) and benign pleural disease (BPD) (n=15). Immunohistochemistry was used to investigate 90K expression in MM and LC tissue sections. The expression of 90K was further evaluated in vitro by ELISA and western blot analysis of conditioned media and cellular extracts of MM, LC and normal human mesothelial (NHM) cell cultures. Finally, the relationships between 90K expression in MM and patient age and survival were studied. The mean 90K level was significantly higher (pp7.3 µg/ml (log rank, p<0.05). This is the first report in MM of the expression of 90K and of its potential diagnostic and prognostic application. Copyright © 2002 John Wiley & Sons, Ltd.