Non‐radioisotopic and Semi‐quantitative Procedure for Terminal Repeat Amplification Protocol
- 1 April 1996
- journal article
- research article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 87 (4) , 329-331
- https://doi.org/10.1111/j.1349-7006.1996.tb00225.x
Abstract
We have used fluorescence‐labeled primers and an auto‐sequencer to detect telomerase activity quickly and easily. The current procedure is superior to the original telomeric repeat amplification protocol in several respects: 1) the result is obtained in real time during electrophoresis, 2) semi‐quantitative results are possible without using a photo‐capture system, and 3) no radioisotope is needed.Keywords
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