>Localization of ribosomal genes in Pleuronectiformes using Ag-, CMA3-banding and in situ hybridization
Open Access
- 5 May 2001
- journal article
- research article
- Published by Springer Nature in Heredity
- Vol. 86 (5) , 531-536
- https://doi.org/10.1046/j.1365-2540.2001.00802.x
Abstract
In this paper we present the analysis of nucleolar organizer regions (NORs) in five species of the order Pleuronectiformes (Scophthalmus maximus, Scophthalmus rhombus, Platichthys flesus, Solea solea and Solea lascaris), a group of fish poorly studied cytogenetically. In spite of the small chromosome sizes, which characterize the karyotypes of this group, we implement both classical (Ag- and CMA3-staining) and molecular (in situ hybridization with a major rDNA probe) techniques for NOR location. NORs were localized in a single chromosome pair in all species studied and showed size variation within each species. Evidence of a NOR-site polymorphism was detected in Platichthys flesus and Scophthalmus maximus. NOR location was apparently the same within families (S. rhombus vs. S maximus, and S. solea vs. S. lascaris) but clearly differed among families, and supports the phylogenetic relationships proposed for the order Pleuronectiformes.Keywords
This publication has 32 references indexed in Scilit:
- Induction of triploidy in the turbot (Scophthalmus maximus)Aquaculture, 2000
- Gene diversity analysis in natural populations and cultured stocks of turbot (Scophthalmus maximus L.)Animal Genetics, 1997
- CMA3-banding pattern and fluorescence in situ hybridiz ation with 18S rRNA genes in zebrafish chromosomesChromosome Research, 1997
- Phylogeny of flatfishes (Pleuronectiformes): comparisons and contradictions of molecular and morpho-anatomical dataJournal of Fish Biology, 1994
- Chromosome??analysis of Bothus podas (Pisces, Pleuronectiformes) from the Mediterranean SeaJournal of Fish Biology, 1993
- Allozyme variation in turbot (Psetta maxima) and brill (Scophthalmus rhombus) (Osteichthyes, Pleuronectoformes, Scophthalmidae) throughout their range in EuropeJournal of Fish Biology, 1992
- Chromosomal NORs as taxonomic and systematic characters in North American cyprinid fishesGenetica, 1988
- The European Garden Flora. Volume 1: Pteridophyta, Gymnospermae, Angiospermae-Monocotyledons (Part I). A Manual for the Identification of Plants Cultivated in Europe, Both Out-of-Doors and Under Glass. S. M. Walters , A. Brady , C. D. Brickell , J. Cullen , P. S. Green , J. Lewis , V. A. Matthews , D. A. Webb , P. F. Yeo , J. C. M. AlexanderThe Quarterly Review of Biology, 1987
- A Method for the Display of Chromosomes of Plaice, Pleuronectes platessa, and Other Marine FishesIchthyology & Herpetology, 1972
- Evolution of Cellular DNA Content in Teleost FishesThe American Naturalist, 1968