Determination of Streptomycin and Dihydrostreptomycin in Animal Tissue by On-Line Sample Enrichment Liquid Chromatography
- 1 March 1994
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 77 (2) , 334-337
- https://doi.org/10.1093/jaoac/77.2.334
Abstract
A method for the determination of streptomycin and dihydrostreptomycin in pork and bovine muscle and kidney was developed. Dilute perchloric acid solution is used to precipitate proteins and extract the analytes from the tissue. The extract is loaded onto a cation-exchange, solid-phase extraction column, and the drugs are eluted with pH 8 phosphate buffer. The eluant is chromatographed by using an on-line column enrichment liquid chromatographic system with postcolumn derivatiza-tion using 1,2-naphthoquinone-4-sulfonic acid and detection by fluorescence. The recoveries were 61.1% (coefficient of variation [CV], 7.3%) for streptomycin and 55.3% (CV, 8.2%) for dihydrostreptomycin. The detection limits were 10 ppb for streptomycin and 20 ppb for dihydrostreptomycin.Keywords
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