Biogenesis of microsomal membrane glycoproteins in rat liver. II. Purification of soluble glycoproteins and their incorporation into microsomal membranes.
Open Access
- 1 December 1975
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 67 (3) , 700-714
- https://doi.org/10.1083/jcb.67.3.700
Abstract
Sialoproteins isolated from the soluble fraction of rat liver could be incorporated into microsomal membranes. This incorporation was dependent on protein concentration, time, and temperature. Sodium dodecyl sulfate gel electrophoresis of membrane proteins after in vitro incorporation showed four major sugar-containing peaks and was similar to that found after in vivo labeling. Most of the incorporated protein was tightly bound to the microsomal membrane. Gel filtration and ion-exchange chromatography revealed the presence of several cytosolic glycoproteins that could be incorporated into microsomes. During prolonged centrifugation in a KBr solution with a density of 1.21 a highly labeled ([3H]glucosamine) protein (mole wt approximately to 70,000) that was actively incorporated into microsomes could be recovered in the upper region of the tube. These results demonstrate that several cytoplasmic glycoproteins of rat liver are transferred into microsomal membranes and that one of these is a lipoprotein.Keywords
This publication has 15 references indexed in Scilit:
- Turnover of Constituents of the Endoplasmic Reticulum Membranes of Rat HepatocytesPublished by Elsevier ,2021
- Biogenesis of microsomal membrane glycoproteins in rat liver. I. Presence of glycoproteins in microsomes and cytosol.The Journal of cell biology, 1975
- Transfer of phospholipids between membraneBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1974
- Polypeptide composition of rat high density lipoprotein: Characterization by SDS-gel electrophoresisBiochemical and Biophysical Research Communications, 1974
- A Molecular Model of High Density LipoproteinsProceedings of the National Academy of Sciences, 1974
- Membrane glycoproteins of the rat small intestine: Chemical composition of membrane glycoproteinsBiochimica et Biophysica Acta (BBA) - Protein Structure, 1974
- Biosynthesis of plasma lipoproteins.1971
- The Metabolism of Glycoproteins of Rat Liver Microsomes*The Journal of Biochemistry, 1970
- Control of Enzyme Levels in Animal TissuesAnnual Review of Biochemistry, 1970
- Intracellular Localization of Liver Sugar Nucleotide Glycoprotein Glycosyltransferases in a Golgi-rich FractionJournal of Biological Chemistry, 1970