Abnormal cytolytic activity of lymphocyte function-associated antigen-1-deficient human cytolytic T lymphocyte clones.
Open Access
- 1 November 1986
- journal article
- research article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 78 (5) , 1387-1391
- https://doi.org/10.1172/jci112725
Abstract
The involvement of the lymphocyte function-associated antigen-1 (LFA-1) membrane molecule in cytolytic T lymphocyte (CTL) interactions with lymphoid target cells was investigated using CTL clones derived from two patients with a heritable deficiency of LFA-1. LFA-1 surface expression on the CTL clones was 1% of the normal level of LFA-1, unchanged with prolonged culture, and identical on 14 different CTL clones. The function of the LFA-1 molecule was addressed using the LFA-1-deficient CTL clones and LFA-1-deficient lymphoid target cells. The lytic activity of the LFA-1-deficient CTL clones was 43% of control when tested against a target cell line expressing normal levels of LFA-1 and less than 10% of control when tested against an LFA-1-deficient target cell line. These results demonstrate a direct involvement of LFA-1 in CTL-mediated cytolysis and suggest a more general dependence on LFA-1 in lymphoid cell-cell interactions.This publication has 17 references indexed in Scilit:
- Interferon-gamma induction of LFA-1-mediated homotypic adhesion of human monocytes.The Journal of Immunology, 1986
- LFA-1 membrane molecule in the regulation of homotypic adhesions of human B lymphocytes.The Journal of Immunology, 1985
- Immunologic studies with LFA-1- and Mo1-deficient lymphocytes from a patient with recurrent bacterial infections.The Journal of Immunology, 1985
- Inherited deficiency of the Mac-1, LFA-1, p150,95 glycoprotein family and its molecular basis.The Journal of Experimental Medicine, 1984
- Deficiency of a leukocyte surface glycoprotein (LFA-1) in two patients with Mo1 deficiency. Effects of cell activation on Mo1/LFA-1 surface expression in normal and deficient leukocytes.Journal of Clinical Investigation, 1984
- LFA-1, LFA-2, and LFA-3 antigens are involved in CTL-target conjugation.The Journal of Immunology, 1984
- A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.The Journal of Experimental Medicine, 1983
- Human lymphocyte function associated antigen-1 (LFA-1): identification of multiple antigenic epitopes and their relationship to CTL-mediated cytotoxicity.The Journal of Immunology, 1983
- The functional significance, distribution, and structure of LFA-1, LFA-2, and LFA-3: cell surface antigens associated with CTL-target interactions.The Journal of Immunology, 1983
- Monoclonal antibody to a human histocompatibility alloantigen, HLA-A2Nature, 1978