Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model
- 1 April 1997
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 35 (4) , 995-8
- https://doi.org/10.1128/jcm.35.4.995-998.1997
Abstract
A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet.Keywords
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