Monoclonal Antibodies to the Human γ2 Subunit of the GABAA/Benzodiazepine Receptors
- 1 March 1995
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 64 (3) , 1305-1311
- https://doi.org/10.1046/j.1471-4159.1995.64031305.x
Abstract
The large intracellular loop (IL) of the gamma 2 subunit of the cloned human gamma-aminobutyric acidA (GABAA) receptor (gamma 2 IL) was expressed in bacteria as glutathione-S-transferase and staphylococcal protein A fusion proteins. Mice were immunized with the fusion proteins (one protein per animal), and monoclonal antibodies were obtained. Six monoclonal antibodies reacted with the gamma 2 IL moiety of the fusion proteins. Three of these monoclonal antibodies also immunoprecipitated a high proportion of the GABAA/benzodiazepine receptors from bovine and rat brain and reacted with a wide 44,000-49,000-M(r) peptide band in immunoblots of affinity-purified GABAA receptors. These monoclonal antibodies are valuable reagents for the molecular characterization of the GABAA receptors in various brain regions.Keywords
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