MIGRATION-INHIBITION OF T-LYMPHOCYTES FROM HUMAN PERIPHERAL-BLOOD BY SPECIFIC ANTIGEN AND LYMPHOKINES

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 32  (2) , 333-338
Abstract
The inhibition of migration of human peripheral blood cells in the presence of PPD [purified protein derivative] was studied. Migration inhibition of peripheral blood mononuclear cells (MN) from Mantoux-positive donors was far greater than the migration inhibition of peripheral blood leukocytes (PBL). Moreover, MN cells and T [thymus-derived] lymphocytes showed larger and more uniform areas of migration. In contrast, the migration of B [bone marrow-derived] lymphocytes and monocytes was poor. Further analysis using purified subpopulations of MN cells showed that PPD inhibited the migration of T lymphocytes but not of B lymphocytes and monocytes. Corresponding to these findings, lymphokine-containing supernatants also inhibited the migration of purified T cells from Mantoux-negative donors. The T lymphocyte was probably the predominant cell in the MN cell population which migrated, and was subject to inhibition by PPD or lymphokines. The movement of human T lymphocytes may be influenced by soluble factors from antigen-activated sensitized cells.