The hydrogen ion dissociation curve of the crystalline albumin of the hen's egg

Abstract
Data are submitted for the construction of a H-ion dissociation curve of egg albumin. An error not greater than [plus or minus] 0.5 equiv. of H per gm. mol (34,500 gm.) of albumin is claimed within the range pH 2-11.5. A maximum H-ion combining capacity of 30-32 equiv. per gm. mol is attained near pH 2, but no maximum dissociating capacity has been observed around pH 11.5-12.0. A method of crystallizing egg albumin using Na2SO4 as a salting-out agent is described.

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