ASSESSMENT OF ANTI-TUMOR CELL EFFECT OF HUMAN-LEUKOCYTE INTERFERON IN COMBINATION WITH ANTICANCER AGENTS BY A CONVENIENT ASSAY SYSTEM IN MONOLAYER CELL-CULTURE

  • 1 January 1982
    • journal article
    • research article
    • Vol. 73  (4) , 667-674
Abstract
Cell growth inhibition by anticancer agents was determined by a simple dye-uptake method. This technique could be performed more easily and rapidly than the cell counting method, the method for measurement of 3H-thymidine incorporation or other dye-uptake methods. The number of viable cells was linearly correlated with the amount of dye taken-up the surviving cells, so that the method described in this article could be applied to the assessment of antitumor cell effects of human leukocyte interferon (Hu IFN-.alpha.) in combination with anticancer agents. In combinations of Hu IFN-.alpha. with anticancer agents such as bleomycin, mitomycin C and cytosine arabinoside in certain concentration ranges, antagonism was noted when Hu IFN-.alpha. and anticancer agents were added simultaneously to the cell culture [human epidermoid carcinoma KB cells]. Outside those ranges of concentration, additive effects were observed. The treatment of the cell with IFN followed by the anticancer agents and vice versa showed additive effects.