hUBC9 associates with MEKK1 and type I TNF‐α receptor and stimulates NFκB activity

3 April 1998

journal article
Published by Wiley in FEBS Letters

Vol. 425 (3) , 431-435
https://doi.org/10.1016/s0014-5793(98)00287-7

Abstract

HUBC9, an E2 ubiquitin conjugating enzyme, was identified by yeast two‐hybrid screening and coprecipitation studies to interact with MEKK1 and the type I TNF‐α receptor, respectively. Because both of these proteins regulate NFκB activity, the role of hUBC9 in modulating NFκB activity was investigated. Overexpression of hUBC9 in HeLa cells stimulated the activity of NFκB as determined by NFκB reporter and IL‐6 secretion assays. hUBC9 also synergized with MEKK1 to activate NFκB reporter activity. Thus, hUBC9 modulates NFκB activity which, at least in part, can be attributed to its interaction with MEKK1 and the type I TNF‐α receptor.

Keywords

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