Evaluation of Media and Reagents for Indole-spot Tests in Anaerobic Bacteriology
- 1 September 1972
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 58 (3) , 335-338
- https://doi.org/10.1093/ajcp/58.3.335
Abstract
Indole-spot tests for use in anaerobic bacteriology were evaluated. Spot test results obtained with four reagents and 143 anaerobic organisms grown on blood agars prepared with three commonly-used bases were compared with those obtained with a tube test utilizing indole-nitrate medium and Kovacs’ test. Correlation of spot test with tube test results was best when organisms were grown on trypticase soy blood agar and tested with 1% p-dimethylaminocinnamaldehyde in 10% (v/v) concentrated hydrochloric acid. This indole-spot test offers a simple, reliable, economical, and rapid method for detection of indole production with anaerobic bacteria.Keywords
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