DNA analysis of intracellular campylobacter-like organisms associated with the porcine proliferative enteropathies: novel organism proposed
- 1 June 1990
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 69 (3) , 189-193
- https://doi.org/10.1016/0378-1097(90)90063-v
Abstract
Intracellular Campylobacter-like organisms are a consistent feature of the porcine proliferative enteropathies. The relationship between these organisms and known Campylobacter sp. previously associated with the disease was studied using restriction enzyme analysis and DNA-DNA blot hybridization techniques. BglII restriction enzyme fragment patterns of DNA of the Campylobacter- like organisms were fundamentally different from those of C. mucosalis, C. hyointestinalis, C. jejuni, and C. coli Crude DNA preparations from Campylobacter-like organisms hydridized strongly with homologous preparations, weakly with porcine DNA and not at all with DNA from Campylobacter sp. Fragment specific DNA probes prepared from Campylobacter-like organisms only hybridized with homologous preparations. This work suggests that the intracellular Campylobacter-like organisms are not one of the known Campylobacter sp. It is possible that they are a novel, uncultured organism worthy of a new name, such as ‘C. intracellulare’.Keywords
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