A VERSATILE HIGH-VOLTAGE PAPER ELECTROPHORESIS APPARATUS AND ITS APPLICATION TO COMPLEX BIOLOGICAL MATERIALS
- 1 August 1961
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 39 (8) , 1313-1324
- https://doi.org/10.1139/o61-138
Abstract
Improvements on a high-voltage paper electrophoresis apparatus are described. The major improvements consist of a simplified cooling system and modification of the cooling plate to accommodate filter paper sheets 24 × 48 in. in size. Two methods, both employing solid heat exchangers, have been devised for the dissipation of Joule's heat: (a) external cooling, employing a Freon 12 circulating coolant and (b) no external cooling, but with heat conductance and radiation by means of an aluminum plate and metal rail.The application of this modified apparatus to the separation of amino acids and peptides from complex biological materials is illustrated.Keywords
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