Notes on Technic: “Clearing” Steel Knife Epon Sections in a Polystyrene Film Sandwich

1 January 1976

journal article
research article
Published by Taylor & Francis in Stain Technology

Vol. 51 (2) , 139-140
https://doi.org/10.3109/10520297609116686

Abstract

Serial sectioning epoxy embedments by steel knife permits rapid light microscope survey of large tissue volumes, and preselection of areas of interest for electron microscopy. Acetate film (Hollander 1970) and Turtox plastic slides (West 1972) have been suggested as substrates upon which the sections may be “cleared” with an added layer of cured epoxy. In our experience, these substrates are excessively adherent to Epon, and “cleared” sections thinner than 40-50 μm cannot be released from them reliably. The following method is suitable for processing Epon sections 10 or more microns thick.

This publication has 2 references indexed in Scilit:

Superficial Warming of Epoxy Blocks for Cutting of 25–150 μm Sections to be Resectioned in the 40–90 nm Range
Stain Technology, 1972
The section embedding (SE) technique. A new method for the combined light microscopic and electron microscopic examination of central nervous tissue
Brain Research, 1970