Transport of phenylalanine by conidia of Fusarium sulphureum
- 1 September 1976
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 22 (9) , 1390-1396
- https://doi.org/10.1139/m76-203
Abstract
L-Phenylalanine was actively transported by conidia of Fusarium sulphureum Schlect (isolate 1). Uptake was optimal at pH 7, 30 °C; respiration-dependent; and was unaffected by relatively high internal concentrations of phenylalanine. The Km for transport was 1–3 × 10−5 M and the Vmax was 2.5–4 nmol/min per milligram dry weight. Phenylalanine is transported by a general transport system for basic and neutral amino acids. Sucrose repressed uptake of phenylalanine and this repression was largely negated by cycloheximide. Efflux of accumulated phenylalanine was influx-dependent; this transport system deteriorated slowly with aging of the conidial culture.This publication has 0 references indexed in Scilit: