Analysis of the transforming potential of the human H-ras gene by random mutagenesis.

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Abstract
Some tumor cells contain mutant ras genes that are capable of transforming NIH 3T3 cells. Those genes that were analyzed arise from the wild-type, non-transforming ras genes by mutations producing single amino acid substitutions at position 12 or 61 of the encoded protein. Random bisulfite-induced mutagenesis was performed on the cloned wild-type human H-ras gene to find if mutations at other positions can activate the transforming potential of that gene. Most mutations are not activating, but mutations that specify single amino acid substitutions at position 12, 13, 59, or 63 if the encoded protein do activate the transforming potential of the H-ras gene. Some, but not all, mutant ras proteins show an altered electrophoretic mobility in NaDodSO4[sodium dodecyl sulfate]/polyacrylamide gels.