Low-molecular-weight peptide inhibits RNA synthesis in human leukemic and phytohemagglutinin-stimulated leukocytes and globin mRNA transcription in differentiating Friend cells.

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Abstract
RNA synthesis of human leukemic leukocytes and phytohemagglutinin-stimulated lymphocytes is reduced by administration of a low-molecular-weight nonhistone peptide factor from calf thymus. Treatment with the factor inhibits Hb production and globin mRNA transcription in dimethyl sulfoxide-stimulated Friend mouse leukemia cells without appreciably modifying the rate of cell growth. Evidence for specificity of these effects is provided by the lack of action of the factor on growth rate and RNA synthesis of a number of nondifferentiating cell lines from various animal species. After removal of the compound, human lymphocytes and Friend cells can be stimulated by phytohemagglutinin and dimethyl sulfoxide, respectively, ruling out any toxic effect.