Detection of gelatinolytic enzyme activities after sodium dodecyl sulfate‐electrophoresis and protein blotting
- 1 January 1991
- journal article
- Published by Wiley in Electrophoresis
- Vol. 12 (6) , 444-445
- https://doi.org/10.1002/elps.1150120613
Abstract
Visualization of proteases with gelatinolytic activity in sodium dodecyl sulfate gels is described. After conventional sodium dodecyl sulfate‐polycrylamide gel electrophoresis, proteins are transferred onto nitrocellulose membranes preincubated with 0.3% gelatine. During the protein electrotransfer, the proteases are renaturated and their enzymatic activity is restored. After nonspecific protein staining, bands with proteolytic activity appear as white areas on a dark background.Keywords
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