Regulation and Physiological Significance of ClpC and ClpP in Streptococcus mutans

Publisher Website
Google Scholar
Abstract
Tolerance of environmental stress, especially low pH, by Streptococcus mutans is central to the virulence of this organism. The Clp ATPases are implicated in the tolerance of, and regulation of the response to, stresses by virtue of their protein reactivation and remodeling activities and their capacity to target misfolded proteins for degradation by the ClpP peptidase. The purpose of this study was to dissect the role of selected clp genes in the stress responses of S. mutans , with a particular focus on acid tolerance and adaptation. Homologues of the clpB , clpC , clpE , clpL , clpX , and clpP genes were identified in the S. mutans genome. The expression of clpC and clpP , which were chosen as the focus of this study, was induced at low pH and at growth above 40°C. Inactivation of ctsR , the first of two genes in the clpC operon, demonstrated that CtsR acts as a repressor of clp and groES-EL gene expression. Strains lacking ClpP, but not strains lacking ClpC, were impaired in their ability to grow under stress-inducing conditions, formed long chains, aggregated in culture, had reduced genetic transformation efficiencies, and had a reduced capacity to form biofilms. Comparison of two-dimensional protein gels from wild-type cells and the ctsR and clpP mutants revealed many changes in the protein expression patterns. In particular, in the clpP mutant, there was an increased production of GroESL and DnaK, suggesting that cells were stressed, probably due to the accumulation of denatured proteins.