Myristic acid is the NH2‐terminal blocking group of the 43‐kDa protein of Torpedo nicotinic post‐synaptic membranes
- 16 January 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 243 (1) , 65-69
- https://doi.org/10.1016/0014-5793(89)81219-0
Abstract
The NH2‐terminal blocking group of the 43‐kDa peripheral membrane protein (43‐kDa protein) of Torpedo post‐synaptic membranes has been identified as myristic acid. To identify that blocking group pure 43‐kDa protein was digested with trypsin and the blocked tryptic peptide was isolated by reverse phase HPLC. That peptide coeluted with and had the same amino acid composition as a synthetic peptide, myristoyl‐Gly‐Gln‐Asp‐Gln‐Thr‐Lys, the structure of the amino terminus predicted from the protein sequence deduced from a cDNA clone. The presence of myristate was confirmed by the precise molecular mass of the peptide, 886.5266, determined by fast atom bombardment mass spectroscopy.Keywords
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