A Rapid Detection and Identification of Hemorphins Released from Bovine Hemoglobin Enzymatic Hydrolysis by Use of HPLC Coupled with Photodiode Array Detector
- 1 January 1995
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 18 (1) , 93-103
- https://doi.org/10.1080/10826079508009223
Abstract
Identification of hemorphins issued from a complex hemoglobin enzymatic hydrolysate was carried out by UV-spectra comparison. Two hemorphins, VV-hemorphin-7 and LVV-hemorphin-7, were detected in a single step by the use of HPLC coupled with photodiode array detector. This technique greatly simplified the the multistage identification and purification strategy. This method could also be efficiently applied to the identification of peptides containing aromatic amino acids.Keywords
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