Fructose-induced adenine nucleotide catabolism in isolated rat hepatocytes

Publisher Website
Google Scholar
Abstract
The mechanism of fructose-induced nucleotide catabolism was studied using isolated rat hepatocytes in which the adenine nucleotide pool was prelabeled with [14C]adenine. Incubation of these cells with fructose caused a rapid depletion of the adenine nucleotides and a corresponding increase in allantoin. There was no accumulation of radioactivity in adenosine in the presence or absence of the adenosine deaminase inhibitor 9-erythro-(2-hydroxy-3-nonyl)adenine. This confirmed the previous hypothesis that fructose-induced adenine nucleotide catabolism occurred by way of AMP deaminase (AMP amino-hydrolase, EC 3.5.4.6).