Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei
Open Access
- 19 March 2008
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 147 (1) , 30-40
- https://doi.org/10.1104/pp.107.115246
Abstract
We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.Keywords
This publication has 42 references indexed in Scilit:
- Functional Sieve Element ProtoplastsPlant Physiology, 2007
- Deficiency in Phosphatidylserine Decarboxylase Activity in the psd1 psd2 psd3 Triple Mutant of Arabidopsis Affects Phosphatidylethanolamine Accumulation in MitochondriaPlant Physiology, 2007
- Sphingolipids and cell deathApoptosis, 2007
- Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cellsBMC Genomics, 2007
- Transcriptional and posttranscriptional regulation of transcription factor expression in Arabidopsis rootsProceedings of the National Academy of Sciences, 2006
- Cell type–specific expression profiling in plants via cell sorting of protoplasts from fluorescent reporter linesNature Methods, 2005
- BiNGO: a Cytoscape plugin to assess overrepresentation of Gene Ontology categories in Biological NetworksBioinformatics, 2005
- Immunopurification of Polyribosomal Complexes of Arabidopsis for Global Analysis of Gene ExpressionPlant Physiology, 2005
- Phosphoenolpyruvate carboxylase kinase is a novel protein kinase regulated at the level of expressionThe Plant Journal, 1999
- Evaluation in tobacco of the organ specificity and strength of therolD promoter, domain A of the 35S promoter and the 35S2 promoterTransgenic Research, 1995